Miroslav Mydlík, Katarína Derzsiová, Katarína Javorská, Katarína Šebeková, Ivana Koborová, Radana Gurecká
End stage renal disease (ESRD) is characterized (among others) by enhanced microinflammation, oxidative- and carbonyl-
stress.The aim of the study was to investigate the effect of haemodiafiltration (HDF) on the variables associated
with the above mentioned conditions in patients (pts) with (DM) or without diabetes mellitus (nDM). In 7pts with DM
(age: 55 ± 14 yrs) and 14 nDM pts (age: 63 ± 12 yrs) except standard biochemical parameters, plasma advanced glycation
end-products (AGEs) by spectrofluorometric method, plasma Nε-carboxymetyllysine (CML) was determined by ELISA
kit MicroCoat, Bernried, Germany, advanced oxidation protein products (AOPPs), total antioxidant capacity (TAC),
fructosamine (FTA), and ferric reducing ability of plasma (FRAP) by photometric methods, tissue inhibitor of metalloproteinases-
1 (TIMP-1) and monocyte chemoattractant protein-1 (MCP-1) by ELISA methods were determined. DM and
nDM groups differed significantly in the pre-HDF AGEs/Alb (19.5 ± 3.3 resp. 23.0 ± 4.7 AU/g, p<0.05), CML/Alb (94.3 ±
30.1 resp. 118.8 ± 55.5 μg/g, p < 0.05) and TIMP-1 (144.5 ± 49 resp. 203 ± 87 ng/mL, p < 0.01); and in the post-HDF FRAP
(706 ± 233 resp. 466 ± 151 μmol/L, p<0.05) levels. In comparison with basal values post-HDF levels of AGEs/Alb, TAC
and FRAP were significantly lower in both groups, while those of FTA, TIMP-1 and MCP-1 did not differ significantly.
Our data suggest that in HDF treated ESRD patients presence of DM modulates some markers of oxidative-and carbonyl-
stress and those of microinflammation. In both subgroups HDF ameliorates only some of the investigated parameters.
The effects of HDF were more pronounced in the nDM patients. Paradoxical finding of lower pre-HDF levels of AGEs/
Alb-associated fluorescence of plasma and plasma CML/Alb, and TIMP-1 in the DM vs. nDM pts remains unclear, but
might reflect the adherence of the DM patients to the recommended diet.