Eva Weismanová, Anna Čukášová. Miriam Vizváryová, Michal Konečný, Peter Weismann
The human papillomaviruses with high oncogenic risk (HR-HPV) are established to be the etiological factor of the development of invasive cervical cancer and its precursors. In transient infection the HR-HPV genome remains circular and the competent immune system is able to clear viral infection within 24 months. Persistent infection is characterized by HR-HPV genome integration into host cell genome, E6 and E7 oncogene expression and development of high-grade cervical intraepithelial neoplasia (CIN). By using molecular methods we detected E6 and E7 oncogene expression in patients with ASCUS and CIN 1, so the occult high-grade CIN was revealed. We suggested the algorithm for molecule-biological methods used in the early CIN progression detection. We emphasized the importance of new biological markers establishment in cytology and histopathology.